Generator

Part:BBa_K318000:Design

Designed by: Nathaniel Pantalone, Justin Vrana   Group: iGEM10_Wisconsin-Madison   (2010-08-27)


pBad + Cre + pLac + Hin + pCons + lacI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1252
    Illegal NheI site found at 3141
    Illegal NheI site found at 3164
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4342
    Illegal BamHI site found at 1191
    Illegal BamHI site found at 1642
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1026
    Illegal AgeI site found at 1328
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1008


Design Notes

Cells transformed with this part tend to grow slowly because of J23100.


Source

Registry

References